Bacteroides Bile Esculin Agar Base (BBE)

Bacteroides Bile Esculin Agar Base (BBE) from Sri Science India Scientific is a dehydrated selective culture medium formulated for the isolation and presumptive identification of Bacteroides fragilis group organisms from food and clinical specimens. The base contains bile salts for selective suppression of Gram-positive and non-bile-tolerant anaerobes, esculin as the differential substrate, and ferric ammonium citrate as the indicator system yielding a blackening reaction upon esculin hydrolysis. Supplied as a 500 g pack under the HiMEDIA and TM MEDIA brand labels, this medium conforms to formulations widely referenced in food safety and pharmaceutical QC microbiology.

The dehydrated base is typically reconstituted at 43.0 g per litre of distilled water, then sterilised and supplemented with antibiotic additives (gentamicin and vancomycin are standard) before pouring plates, though the exact reconstitution rate for this specific pack should be confirmed with the supplier. Bile concentration in BBE formulations is typically 20 g/L, creating a selective pressure that permits Bacteroides fragilis, B. thetaiotaomicron and related species to grow while inhibiting facultative and aerobic contaminants. Esculin hydrolysis by bacterial beta-glucosidase releases esculetin, which complexes with ferric ions to produce a diffuse black precipitate in and around colonies, enabling presumptive identification without additional biochemical testing.

The 500 g pack size suits medium-throughput food microbiology laboratories, clinical anaerobe isolation units and import-export food testing facilities requiring compliance with ISO 15214 or comparable standards for anaerobic bacteria enumeration. Typical end-users include quality-control microbiologists in meat, dairy and ready-to-eat food sectors, as well as hospital laboratories processing peritoneal, blood and deep-tissue specimens for suspected B. fragilis group sepsis. Importers should confirm with Sri Science India Scientific the manufacturing batch certificate of analysis, storage condition on dispatch from Chennai, and whether the product is shipped with indicated expiry and dry-condition guarantee.

Sri Science India Scientific operates from 7/63, Macmillan Colony 3rd Street, Nanganallur, Chennai, Kanchipuram, Tamil Nadu 600061, India. The company is listed at Harbor trust tier but shows a 0.00 percent response rate with zero average response hours, which means prospective buyers should not assume rapid quotation or technical support without direct follow-up by telephone or alternate channels.

The supplier positions itself as a distributor of HiMEDIA and TM MEDIA branded dehydrated culture media rather than as a manufacturer. No founding year, staff count, revenue, ISO certification status, or client list appears in the source data. Buyers should verify whether Sri Science India Scientific holds a current wholesale drug licence or equivalent, whether they maintain cold-chain storage in Chennai for temperature-sensitive media, and whether they can provide manufacturer batch records and certificates of analysis with each 500 g pack.