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The One-step Probe RT-qPCR kit supplied by Dongsheng Biotech Co., Ltd combines a hot‑start DNA polymerase, reverse transcriptase, probe mix, primers, dNTPs and a buffer solution in a single‑tube mastermix. It is designed to detect the SARS‑CoV‑2 ORF1ab and N gene sequences together with an internal control targeting the human RNase P gene. The kit is marketed for nucleic acid detection in nasopharyngeal swabs, oropharyngeal swabs, sputum and other clinical specimens. All reagents are provided in a ready‑to‑use format that eliminates separate reverse‑transcription steps.
The enzyme formulation uses a hot‑start polymerase that activates at 95 °C, providing high specificity and reducing non‑specific amplification. Reverse transcriptase activity enables conversion of viral RNA to cDNA within the same reaction, shortening turnaround time for diagnostic laboratories. Dongsheng Biotech states that the product holds CE‑IVD and ISO 13485 certifications, aligning it with European in‑vitro diagnostic standards. Typical reaction volumes are 20 µL and the assay follows a standard thermal profile of 50 °C for 10 min, 95 °C for 2 min and 40 cycles of 95 °C for 15 s and 60 °C for 1 min.
Importers and diagnostic kit distributors source this kit for bulk supply to clinical laboratories. The listing does not disclose minimum order quantities, lead times or specific Incoterms, so buyers should request those details directly from Dongsheng Biotech. Certification copies, batch‑specific certificates of analysis and storage requirements must be confirmed before shipment. The product is shipped in cryogenic vials, each typically containing 25 reactions, and requires cold‑chain logistics to maintain reagent activity.
| Material | Enzyme mastermix containing hot‑start DNA polymerase, reverse transcriptase, probe mix, primers, dNTPs and buffer solution (as supplied) |
| Target Genes | SARS‑CoV‑2 ORF1ab and N gene sequences (specified) |
| Internal Control | Human RNase P gene assay (included) |
| Certification | CE‑IVD certified (as declared) |
| Quality System | ISO 13485 certified (as declared) |
| Typical Reaction Volume | 20 µL per assay (typical) |
| Typical Thermal Profile | 50 °C 10 min reverse transcription, 95 °C 2 min activation, 40 cycles of 95 °C 15 s / 60 °C 1 min (typical) |
| Storage Condition | -20 °C (typical) |
| Typical Shelf Life | 12 months when stored at -20 °C (typical) |
| Packaging Format | Cryogenic vials containing 25 reactions each (typical) |
Dongsheng Biotech Co., Ltd is located at #a‑305, Basigo Intelligent Park, no.179 Guangpu East Rd, Huangpu District, Guangzhou, Guangdong 510663, China. The supplier is listed with a harbour‑tier verification and currently shows a 0 % response rate, indicating that no response data have been recorded on the platform. The company specialises in in‑vitro diagnostic reagents and offers CE‑IVD and ISO 13485 certified kits for molecular detection of viral pathogens.
The product page confirms that the One‑step Probe RT‑qPCR kit complies with European diagnostic regulations, but no further corporate history, employee numbers or client references are provided. Buyers should treat the listed certifications as the primary evidence of quality and request additional documentation such as a Certificate of Free Sale or a recent audit report before finalising a purchase.
One-step Probe RT-qPCR kits are single-tube molecular diagnostic reagents combining reverse transcription and quantitative PCR for rapid nucleic acid detection. These kits are engineered for high sensitivity and specificity in detecting viral targets such as SARS-CoV-2 ORF1ab and N gene sequences. Ideal for clinical laboratories and diagnostic kit distributors seeking CE-IVD and ISO 13485 certified products for bulk import and distribution in the in vitro diagnostics market.
| Business Type | Supplier |
| Year Established | Recently Joined |
| Employees | Contact Supplier |
| Annual Revenue | Contact Supplier |
| Main Products | View Products Tab |
| Major Markets | Global |
| Response Time | Contact Supplier |
| Response Rate | New Supplier |
Before placing an order, importers should obtain copies of the CE‑IVD and ISO 13485 certificates, as well as a batch‑specific Certificate of Analysis. Verifying the expiration date of the reagents and confirming the target gene sequences match the diagnostic guidelines of the destination market are essential steps to avoid regulatory delays.
Logistics for this product require temperature‑controlled transport. Buyers need to ask the supplier which Incoterms are offered (e.g., FOB Shanghai, CIF) and what the expected lead time is, since the listing does not disclose those details. Payment terms such as T/T or L/C should be clarified in advance, and any required cold‑chain handling fees should be included in the quotation.
Quality assurance should include a third‑party inspection of the manufacturing site, review of the supplier’s quality management system, and sampling of each lot for enzyme activity and probe performance. Importers are advised to request a full inspection report, the original certificates of conformity and, if required, an independent validation of the assay using the supplied internal control. Proper documentation will support regulatory submissions in the buyer’s country.
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When sourcing One-Step Probe Rt-Qpcr Kit for your business, securing the right balance of quality and cost is essential. Dongsheng Biotech Co., Ltd, a verified supplier based in China, offers this product with key specifications including One-step Probe RT-qPCR Kit (single-tube format), Real-time quantitative PCR with fluorescent probe chemistry (e.g., TaqMan), and ORF1ab sequence and N gene of SARS-CoV-2; adaptable to other viral/bacterial targets. By purchasing directly from the manufacturer or authorized exporter, buyers can negotiate favorable FOB prices starting at $0.9/Bags and manage bulk orders with a minimum order quantity (MOQ) of 1 Bags. This product is actively traded under HS Code 3822.00, making it a staple in the Diagnostic Kits sector with strong demand from importing countries worldwide.
Importing One-Step Probe Rt-Qpcr Ki requires careful attention to shipping logistics, customs compliance, and secure payment terms. Dongsheng Biotech Co., Ltd offers flexible shipping options such as Full Container Load (FCL) or Less than Container Load (LCL) via sea freight. To ensure a smooth transaction, buyers should verify import duties for HS Code 3822.00 in their destination country. Common payment methods for international B2B transactions include Letter of Credit (L/C), Telegraphic Transfer (T/T), and Documents against Payment (D/P). All transactions and RFQs are facilitated through EximNext, a leading online B2B marketplace designed to make cross-border trade secure and efficient.
Finding trustworthy partners is the foundation of successful importing. The supplier of this One-Step Probe Rt-Qpcr Ki has been verified on our platform. Whether you are a distributor, wholesaler, or procurement manager, you can request a free quotation, ask for product samples, and finalize your bulk purchase with confidence. EximNext hosts thousands of verified manufacturers and exporters across 200+ countries. Explore similar products in the Diagnostic Kits category and connect with top-tier exporters on our comprehensive B2B marketplace. Start your sourcing journey today.
Importing One-Step Probe Rt-Qpcr Ki requires careful attention to shipping logistics, customs compliance, and secure payment terms. Common shipping options include Full Container Load (FCL) or Less than Container Load (LCL) via sea freight. Buyers should verify import duties for HS Code 3822.00 in their destination country. Common payment methods include Letter of Credit (L/C), Telegraphic Transfer (T/T), and Documents against Payment (D/P).
The current listed wholesale price for One-Step Probe Rt-Qpcr Kit from Dongsheng Biotech Co., Ltd is $0.9/Bags on FOB terms from China. Prices may vary depending on order volume, packaging, and destination. For the most accurate bulk quote, send a direct RFQ to Dongsheng Biotech Co., Ltd through EximNext.
The supplier, Dongsheng Biotech Co., Ltd, has set a Minimum Order Quantity (MOQ) of 1 Bags for One-Step Probe Rt-Qpcr Ki. For sample requests or smaller trial orders, contact the supplier directly through our platform's inquiry system.
For international shipping and customs clearance, One-Step Probe Rt-Qpcr Kit is classified under HS Code 3822.00. Buyers should verify their local customs regulations to determine applicable import duties and taxes for this classification.
To import One-Step Probe Rt-Qpcr Ki from China, negotiate shipping terms (FOB, CIF, or EXW) directly with Dongsheng Biotech Co., Ltd. Ensure you have the necessary import licenses for Diagnostic Kits products in your destination country.
Yes, Dongsheng Biotech Co., Ltd is a verified supplier on EximNext. You can view their complete company profile, business registration details, certifications, and export history before placing a bulk order.
The key specifications for One-Step Probe Rt-Qpcr Kit supplied by Dongsheng Biotech Co., Ltd include One-step Probe RT-qPCR Kit (single-tube format), Real-time quantitative PCR with fluorescent probe chemistry (e.g., TaqMan), and ORF1ab sequence and N gene of SARS-CoV-2; adaptable to other viral/bacterial targets. For a complete technical data sheet or Certificate of Analysis (COA), send an inquiry directly to the supplier through EximNext.
Dongsheng Biotech Co., Ltd offers One-Step Probe Rt-Qpcr Ki with standard export packaging. Custom packaging, private labeling, and OEM/ODM options may also be available for large wholesale orders.
One-Step Probe Rt-Qpcr Ki is actively imported by buyers worldwide. Request a destination-specific CIF or CFR quote from Dongsheng Biotech Co., Ltd through our platform.
Dongsheng Biotech Co., Ltd maintains international quality certifications. These ensure that the One-Step Probe Rt-Qpcr Ki meets international quality, safety, and regulatory standards required for cross-border trade.
Click "Request Quotation" on this product page, specify your required quantity, preferred shipping terms, and destination country. Dongsheng Biotech Co., Ltd will respond with a detailed wholesale quote including FOB pricing, lead time, and payment options.
Common payment methods include Letter of Credit (L/C), Telegraphic Transfer (T/T), Documents against Payment (D/P), and Escrow services. Confirm accepted terms directly with Dongsheng Biotech Co., Ltd.
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