Introduction: 4-hydroxyethyl piperazine ethosulfonic acid (HEPES) is an important hydrogen ion buffer, which has a good buffer ability in the pH range of 6.8 to 8.2, and can control a constant pH for a long time. Using a concentration of 10 ~ 50mmol/L, the general culture medium containing 20mmol/L 4-hydroxyethyl piperazine taurine can have a good buffer capacity, and has no toxic effect on cells. Application: HEPES (4-hydroxyethyl piperazinethesulfonic acid) is a non-ionic amphoteric buffer with high polarity, and is inert to a variety of chemical reagents and enzymes. It does not participate in and interfere with the process of biochemical reactions, and has no inhibitory effect on enzyme chemical reactions. Therefore, it can be used specifically for the research of proteins and enzymes that are highly volatile and ph-sensitive to organelles and very volatile. Buffer solution preparation: (1)hepes buffer can be directly added to the prepared culture medium according to the desired concentration, and then filtered to remove bacteria. 2.38 g HEPES was added to each 1000ml of culture medium, the pH was adjusted to 7.2 with lNNaOH after dissolution, and the bacteria were removed by filtration before use. At this time, the concentration of HEPES was 10mmol/L. (2) 100x storage solution (lmol/L) could also be prepared. Before use, 99ml culture solution was added to lml storage solution, and the final concentration was 10mmol/L. lmol/L (100x) hepes buffer was prepared as follows: 23.8gHEPES was dissolved in 90ml double distilled water, the pH was adjusted to 7.5-8.0 with lNNaOH, and then the water was fixed to 100ml, filtered to remove bacteria, divided into vials (2ml/ bottle), and stored at 4�ºC or -20�ºC. Purpose: 1.Biological buffer; Reaction buffer, prehybridization buffer and hybridization buffer for isolation and analysis of RNA nuclear fraction; For RNA with T4RNA 2.Molecular biology grade is used for RNA3' -end labeling with T4 RNA ligase 3.Cell-cell adhesion, short-term cell aggregation culture, cleaning of tissue and cell buffer
Buy online Hepes CAS 7365-45-9 Biological Buffer at wholesale price from Hebei Tianao Technology Co., Ltd in China. Hebei Tianao Technology Co., Ltd is the genuine trader of product Hepes CAS 7365-45-9 Biological Buffer and sells it in bulk at affordable and cheapest wholesale price near you. This product is available to import at Exim Next, the best B2B Marketplace for importers and exporters overseas depending upon supplier/seller’s product policies. The Hepes CAS 7365-45-9 Biological Buffer is trending in the market in terms of quality and price list offered by the seller. The availability of product and variations including stock options at our B2B Portal is subject to Hebei Tianao Technology Co., Ltd catalog listing and policies.
At Exim Next, we understand that quality is paramount in the B2B sector. As updated by the seller, the Hepes CAS 7365-45-9 Biological Buffer is manufactured to meet rigorous industry standards, ensuring reliability and performance for your business operations. Designed with businesses in mind, the product offers outstanding end user experience. As your business grows, so do your needs, hence it is designed to scale with you, offering flexible solutions that adapt to changing demands. Explore more such product varieties from sellers, suppliers, manufacturers and exporters at our website. You can even import or export Hepes CAS 7365-45-9 Biological Buffer or connect with global Importers and Exporters online.
Hebei Tianao Technology Co., Ltd is a premier leather products export company, established in 2010 and based in China. With a decade of experience, Hebei Tianao Technology Co., Ltd has built a reputation for delivering high-quality leather goods to clients across the globe. The company specializes in a wide range of leather products, including bags, wallets, belts, and custom leather accessories, all crafted with precision and attention to detail. Leveraging advanced manufacturing techniques and stringent quality control processes, Hebei Tianao Technology Co., Ltd ensures that each product meets the highest standards. The company’s commitment to excellence, timely delivery, and customer satisfaction has made it a trusted name in the international market.Hebei Tianao Technology Co., Ltd continues to expand its global footprint, exporting its superior leather products to various countries, while maintaining strong relationships with clients worldwide.
The trusted supplier, Hebei Tianao Technology Co., Ltd, accepts for transactions related to Hepes CAS 7365-45-9 Biological Buffer. All payments are processed securely via the Exim Next B2B platform, ensuring buyer protection and supplier credibility.
Placing an order for Hepes CAS 7365-45-9 Biological Buffer with Hebei Tianao Technology Co., Ltd is quick and straightforward on Exim Next, a leading B2B marketplace for global buyers and suppliers. You can:
When purchasing Hepes CAS 7365-45-9 Biological Buffer from a B2B marketplace in 2025, focus on the following critical factors:
Comparing prices and communicating directly with verified sellers like Hebei Tianao Technology Co., Ltd can help ensure value for money and better sourcing decisions.
Exim Next is home to a vast network of verified B2B buyers, wholesalers, distributors, and importers of Hepes CAS 7365-45-9 Biological Buffer across industries and countries. To discover the most active and high-value buyers:
Hebei Tianao Technology Co., Ltd, a reliable B2B trader from China, dispatches Hepes CAS 7365-45-9 Biological Buffer primarily through the port at China. Packaging types (e.g., bulk, custom, eco-friendly) and estimated pricing may vary based on order volume and destination. To get detailed logistics and pricing information, reach out via the Inquire Now button or Sign Up for direct supplier interaction.